Transcriptome profiling of rat brain samples in two age groups in responding to intracerebral hemorrhage

Aging-induced decline of endogenous neuroprotection has been shown to aggravate intracerebral hemorrhage (ICH)-induced acute brain injury, however, the underlying mechanisms in brain are still little known. In this study, we applied a rat ICH model to study the transcriptional responses in the early and late aging (13-month and 22-month old) rats that show substantial differences in brain damage and recovery. Transcriptome analysis (RNA-seq) reveals that brain expression of neuroinflammation genes is similarly and selectively upregulated in ICH, which includes genes in the cellular response to interferon gamma function. We show that the anti-IFN-? treatment effectively reduces ICH-induced acute brain injury. Overall design: Rats from two age groups, including 13-month and 22-month, were selected to perform experiments. Left brains of rats were treated with intracerebral hemorrhage, while right brains were as control. Three biological replicates were used to perform RNA-seq experiments. A total of 12 RNA-seq samples were obtained.