MDM2 induces EMT via the B-Raf pathway through 14-3-3

To Study the role of MDM2 in EMT biogenesis and explore the mechanism by which MDM2 promoted EMT in glioma cells, we used whole geneome RNA-seq of U251 cells and stable U251 cells overexpressing MDM2-GFP to study role MDM2 mediated EMT regulation. U251 cells and U251 cells which overexpressing MDM2-GFP were harvested, and then RNA was prepared using the Qiagen RNeasy Kit according to the manufacturer’s instructions. Strand-specific libraries were generated by using the KAPA Stranded mRNA-Seq Kit with KAPA mRNA Capture Beads (KAPA Biosystem Wilmington, MA). After clustering, the Flow Cell was loaded on the Illumina HiSeq instrument according to manufacturer’s instructions.