Proximal tubular cells with different DNA contents single-cell sequencing
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP522147
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资源简介：
To explore the heterogeneity and functional dynamics of proximal tubular cells (PTCs) with different DNA contents, we isolated PTCs (2N, 4N, >4N) from male C57BL/6J mouse kidneys at day 3 and day 14 post-ischemia-reperfusion injury, and generated a single-cell transcriptome atlas.The renal cortices of mice were minced and digested, then the PTCs with different DNA contents were isolated to perform scRNA-seq by fluorescence-activated cell sorting (FACS) based on lotus tetragonolobus lectin (LTL)-positive and DNA content indicated by Hoechst 33342. Library construction was performed according to the instructions of 10x Genomics, and sequencing was conducted using the Illumina HiSeq 4000 system.
创建时间：
2025-02-26
