George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:13400, Xenopus laevis laevis. CIL. Dataset

A normal X. laevis blastomere expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Single microtubules extend to the cortex, even in these large cells. X. laevis embryos were imaged on a laser scanning confocal microscope (1024; Bio-Rad Laboratories) mounted on an Axiovert microscope (Carl Zeiss, Inc.) using a 63× 1.4 NA Plan-Apochromat oil objective. Video corresponds to Supplementary Figure 1D and Video 2 from J Cell Biol. 2009 Dec 14;187(6):831-45.

正常模式下的非洲爪蟾(X. laevis)早期胚胎细胞表达三种串联融合绿色荧光蛋白(GFPs)至微管结合结构域(EMTB-3G；Faire等，1999年)。即使在这些大型细胞中，单个微管也延伸至细胞皮质。X. laevis胚胎在激光扫描共聚焦显微镜(1024；Bio-Rad Laboratories)上成像，该显微镜搭载于Axiovert显微镜（卡尔·蔡司公司）上，并使用63× 1.4 NA Plan-Apochromat油镜。视频与补充图1D和《细胞生物学杂志》(J Cell Biol.) 2009年12月14日第187卷第6期第831-845页中的视频2相对应。