Proteins identified by LC-MS/MS in the apoplast of Arabidopsis thaliana leaves.

*encoded in the chloroplast genome.Apoplastic washing fluids from mock-infected plants were separated by 2-D-gel electrophoresis, picked, trypsinated and used for determination fragment composition by LC-MS/MS. Identification was achieved by data bank queries as outlined under materials and methods. Spot numbers refer to those shown in Fig. 1. S, C, and M indicate predicted signal peptides for the secretory pathway, chloroplasts or mitochondria, respectively. RC indicates reliability classes for the prediction of localization (1 = high, 5 = low). Peptides are shown in Table S1.