Transcriptional regulation of Hepatic Stellate Cell plasticity in NASH

We here perform transcriptional profiling of hepatic stellate cells (HSCs) isolated from Western diet/high fructose-fed C57BL6/J mice, carbon tretrachloride (CCl4)-treated C57BL6/J mice, and of murine HSCs differentiated in vitro. Gene expression profiles from hepatic stellate cells isolated from C57BL6 mice fed a Western Diet supplemented with high fructose for 12, 16 or 24 weeks or normal chow. Gene expression profiles from hepatic stellate cells isolated from C57BL6 mice treated CCl4 for 1, 4 or 8 weeks or treated with vehicle. Gene expression profiles from hepatic stellate cells isolated from C57BL6 mice and seeded on normal plastic cell culture dishes for 1, 4, 8, or 12 days.