ChIP-chip assay on circular chromosome conformation capture (4C) genomic DNA sample from human telomerase-immortalized corneal epithelial (HTCE) cells with the specification of CTCF binding

Ability of CTCF binding on various gene promoters is essential to regulate their expression. It's been demonstrated that cornea-specific Pax6 expression is regulated conversely by CTCF (Li et al.). circular chromosome conformation capture (4C) and ChIP-chip profiling have been performed using HTCE cells. This approach is to elicit the epigenetic mechanism involving CTCF-mediated chromatin remodeling that regulates Pax6 and some target gene interactions. Compare CTCF interactions of gene promoters in HTCE cell