Lung transcript expression profile after Gata5 gene deletion

Gata5 is a zinc finger transcription factor that is expressed in embryonic pulmonary mesenchyme and becomes upregulated in the lungs, gut, and bladder during postnatal development. We used microarray to comapre gene expression profiles of mouse lung between Gata5 knockout and wild type mice. We hope to identify the differentially expressed genes that affected by Gata5 gene deletion and their functional clusters or pathways. The goal of this study is to identify the differentially expressed genes that affected by Gata5 gene deletion and their functional clusters or pathways. Total RNA samples from three Gata5 KO and four wild type control mouse left lungs were extracted from the mouse lungs and profiled using Affymetrix GeneChip Mouse Genome 430 2.0 Array.Gata5是一种锌指转录因子（zinc finger transcription factor），在胚胎肺间质中表达，并在出生后发育阶段于肺、肠道与膀胱内出现表达上调。我们采用微阵列（microarray）技术，比较Gata5基因敲除（Gata5 knockout, KO）与野生型（wild type）小鼠的肺组织基因表达谱。本研究旨在筛选受Gata5基因缺失影响的差异表达基因（differentially expressed genes），并解析其功能聚类或参与的信号通路。我们从3只Gata5 KO小鼠与4只野生型对照小鼠的左肺中提取总RNA样本，采用Affymetrix GeneChip小鼠基因组430 2.0芯片完成表达谱检测分析。