rCRUX Generated MarVer3 Marine Mammal Reference Database
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https://zenodo.org/record/7909663
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资源简介：
rCRUX generated reference database using NCBI nt blast database downloaded in December 2022.
Primer Name: MarVer3 Marine Mammal
Gene: 16S
Length of Target: 232-274
get_seeds_local() minimum length: 160
get_seeds_local() maximum length: 345
blast_seeds() minimum length: 124
blast_seeds() maximum length: 309
max_to_blast: 100
Forward Sequence (5'-3'): AGACGAGAAGACCCTRTG
Reverse Sequence (5'-3'): GGATTGCGCTGTTATCCC
Reference: Valsecchi, E., Bylemans, J., Goodman, S. J., Lombardi, R., Carr, I., Castellano, L., ... & Galli, P. (2020). Novel universal primers for metabarcoding environmental DNA surveys of marine mammals and other marine vertebrates. Environmental DNA, 2(4), 460-476. https://doi.org/10.1002/edn3.72
We chose default rCRUX parameters for get_blast_seeds() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for blast_seeds() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.
创建时间：
2023-10-05
