rCRUX Generated MarVer3 Marine Mammal Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: MarVer3 Marine Mammal Gene: 16S Length of Target: 232-274 get_seeds_local() minimum length: 160 get_seeds_local() maximum length: 345 blast_seeds() minimum length: 124 blast_seeds() maximum length: 309 max_to_blast: 100 Forward Sequence (5'-3'): AGACGAGAAGACCCTRTG Reverse Sequence (5'-3'): GGATTGCGCTGTTATCCC Reference: Valsecchi, E., Bylemans, J., Goodman, S. J., Lombardi, R., Carr, I., Castellano, L., ... & Galli, P. (2020). Novel universal primers for metabarcoding environmental DNA surveys of marine mammals and other marine vertebrates. Environmental DNA, 2(4), 460-476. https://doi.org/10.1002/edn3.72 We chose default rCRUX parameters for get_blast_seeds() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for blast_seeds() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.