Transcriptomics analysis of C. elegans intestines

To identify new heme responsive genes, we performed transcriptomics analysis on the intestines isolated from worms that were cultured at different concentrations of heme. Results of SMART-seq and gene expression profiling showed that 578 genes were differentially expressed at different heme concentrations. Overall design: Adult worms that had been cultured with 2, 20, or 400 µM heme were paralyzed and dissected. Intestines from three adult worms were combined into one sample. The mRNA profiles were analyzed by SMARTseq, in triplicate, using Illumina NovaSeq 6000 platform.