All concentrations are given per reaction. n.p. qPCR was not performed. a5-virus-mix consists of the HIV-1 strains JR-CSF, NL4-3, HXB2, YU2 and 89.6 (see also Materials and Methods). b3/23 µl of cDNA
The Quantitative Real Time PCR assay was first performed with varying concentrations of primer and then probe to determine the optimal concentration of primers and probe for the assay. The concentrati
High throughput DNA sequencing of bulk invertebrate samples ('metabarcoding') enables simultaneous identification of multiple taxa for environmental monitoring. The accuracy of metabarcoding studies i
