Data downloads for “Functional screens of barcoded expression libraries uncover new gene functions in carbon utilization among gut Bacteroidales”

This figshare includes the data for “Functional screens of barcoded expression libraries uncover new gene functions in carbon utilization among gut Bacteroidales”. For this study, we developed a strategy to barcode expression libraries for high-throughput interrogation of gene functions in competitive fitness assays. We demonstrate the power of this approach to uncover novel phenotypes for uncharacterized genes using pooled libraries constructed from a diverse set of gut Bacteroidales expressed in Bacteroides thetaiotaomicron. We identified new roles in carbohydrate metabolism for nine proteins, including enzymes, transporters, a regulator, and hypothetical proteins from mobile genetic elements.