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TraDIS_Typhimurium_1M, submitted by SC on 20-MAY-13

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https://www.ncbi.nlm.nih.gov/sra/ERP000099
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资源简介:
An estimated 930,000 transposon mutants in S. Typhimurium have been assayed using Illumina nucleotide sequencing from within the transposon into the adjacent target DNA. Using TraDIS (Transposon Directed Insertion-site Sequencing), we have been able to map ~550,000 unique transposon insertion sites to the Salmonella enterica serovar Typhimurium chromosome. This has allowed us to assay simultaneously every gene in the genome for essentiality and generate a genome-wide list of candidate essential genes.
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2021-02-04
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