John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10539, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to raw data stack: CIL# 10538, maximum intensity projection stacks: CIL# 10540 and CIL# 10541 and raw data stack with DIC: CIL# 10543.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

一层人类表皮成纤维细胞感染了表达YFP标记的TgIMC1（内质膜复合支架蛋白）和mCherry标记的人α1微管蛋白的弓形虫Toxoplasma gondii。采用API DeltaVision在Olympus IX70倒置显微镜上获取图像。实验目的：使用Olympus 60X水浸物镜，数值孔径为1.2。滤光片：mCherry，激发波长555-592nm，发射波长600-665nm，YFP，激发波长490-510nm，发射波长525-595nm。图像采集使用CoolSnapHQ/ICX285相机。对应于原始数据堆栈：CIL# 10538，最大强度投影堆栈：CIL# 10540和CIL# 10541，以及带有DIC的原始数据堆栈：CIL# 10543。该图像为未发表图像，与PMID: 16518471发表的图像相似。详见出版物以获取其他实验细节。