Genes targeted and oligonucleotide primers designed.
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*Degenerate primers, where degeneracies follow the IUPAC nomenclature for incompletely specified bases and are described in bold, specifically: ‘Y’ = C or T; ‘K’ = G or T; ‘W’ = A or T; ‘R’ = A or G; ‘H’ = A, C or T. (www.chem.qmul.ac.uk/iubmb/misc/naseq.html).#Primers were 5′ labelled with fluorescent dyes according to the text using either complete dye set A or B.
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2015-12-02
