Characterization of single cysteine substitution mutants P107C-R135C on the background of the basal mutant (C140A/167A/185A/222A264A/316A).
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*The UV/Vis absorbance spectral ratios were determined after elution from the immunoaffinity cloumn at pH 6.0.- The purified mutants P107C, N111C, G114C, A124C, L125C, W126C, L128C, and E134C did not form rhodopsin-like chromophore.#the ratio of rhodopsin mutant E113C was 280∶380.
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2015-12-02
