<i>Giardia Assemblage E</i> isolate P15 Genome Sequence and Annotation

Sequence and annotation of Giardia Assemblage E isolate P15 Genomic DNA was sequenced using a Genome Sequencer FLX/Titanium instrument. Three runs with the Genome Sequencer (454 Life Science) generated 1,639,140 reads. Genome assembly was performed de novo using the sequence assembly software MIRA V2.9.43. Manual inspection and editing of the assembly was carried out using consed and custom Perl scripts. Clustering of the shotgun reads and manual finishing of the assembly resulted in 820 contiguous sequences (contigs) with an N50 of 71,261 bp and an average genome coverage of 47X. The G + C content of the overall assembly is 47%. Gene prediction was performed using GLIMMER 3 and CRITICA. The vast majority of the P15 annotations were assigned using information from the WB and GS genomes.