Human CAR T cells expressing Super2 and IL-33 retain subset identity

Single cell RNAseq analysis was conducted on human TZ47 CAR T cells with or without Super2 and IL-33 co-expression eight days post in vitro manufacturing. Overall design: PBMCs were stimulated for two days starting with anti-CD3 in RPMI complete media with 100 U/ml human IL-2 for 2 days prior to transduction with TZ47 CAR and Super2 + IL33 viral supernatants on sequential days. Human CAR T cells were expanded for a total of 8 days before cells were analyzed by scRNAseq