A targeted RNAi screen identifies 17 RNAi candidates that decrease the prolonged lifespan of the METC mutant isp-1;ctb-1 by at least 10%.

The RNAi candidates identified in the primary screen were retested in the number of independent lifespan assays indicated. The lifespan data from the separate trials were either pooled, compared by a stratified log-rank test (p≤0.001), and the percentage of mean lifespan decrease calculated or compared by log-rank test (p≤0.001) within each trial and the percentage of mean lifespan decrease was averaged. Only the RNAi clones that decrease the lifespan of the isp-1;ctb-1 mutant by at least 10% in the two methods of analysis are represented. Among them, 13 represent transcription factors not previously known to function in aging and four (indicated with asterisks) are transcription factors already known to affect lifespan in C. elegans: HSF-1 [31], NHR-49 [32], DAF-16 [33], and PHA-4 [34]. Quantitative data and statistical analyses for the experiments shown here are included in Table S1.