Effect of 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq data

To investigate the effect of increasing 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq, we labeled 3 different cell lines with increasing concentrations of 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples. Overall design: Increasing labeling concentrations (up to 800 µM) in U2OS, HCT116 and HFF-TerT cells for up to 2 hours in 2 replicates.