Spore viability and genetic map distances in EAY1108/EAY1112 strains bearing the indicated <i>msh4</i> and <i>msh5</i> mutations.

Percent spore viability and the genetic map distance (sum of four genetic intervals, URA3-LEU2, LEU2-LYS2, LYS2-ADE2, ADE2-HIS3; [24]) from single spores are shown for each of the fifty-seven msh4 and msh5 alleles. Amino acid substitutions indicate the wild-type residue, amino acid position, mutation. The different classes indicate; 1: amino acids conserved in Msh4 and Msh5 in five species (S. cerevisiae, A. thaliana, C. elegans, M. musculus and H. sapiens) but absent in S. cerevisiae Msh2, Msh3, Msh6. 2: amino acid residues conserved in Msh4 only across five species. 3: amino acid residues conserved in MSH5 only across five species. 4: amino acid residues conserved in Msh4 and Msh5 across five species as well as in S. cerevisiae Msh2, Msh3 and Msh6. Mutations were also mapped with respect to specific domains in Taq MutS. msh4-G639A and msh5-G648A are analogous to Msh2 ATP binding mutations [55]. msh4-R676W and msh5-R685W are analogous to Msh2 and Msh6 ATP hydrolysis mutations [53]. Recombination frequencies (recombinant spores/total spores) were multiplied by 100 to obtain genetic map distance in centimorgans (cM). The total number of tetrads dissected (n) for each mutant is shown. Wild-type and msh5Δ data are from Argueso et al.[24]. Yeast two-hybrid analysis was performed for the indicated msh4 and msh5 mutants. β-galactosidase activity (Miller units ± standard deviation) from three independent co-transformants involving the msh4 and msh5 mutants and the corresponding wild-type MSH4 or MSH5 partner is shown.