Genome-wide analysis of cleavage sites in Lactuca sativa L. cv. Green wave

In previous studies, high-throughput approaches for genome-wide profiling of RNA cleavage sites were developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3'gene ; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) using 30 days after germination of Lactuca sativa L. to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.