Gene expression profile of colonic goblet cells (GCs) from SpdefKO_RedMUC298trTg and RedMUC298trTg mice

Spdef is a transcription factor involved in differentiation of goblet cells. Goblet cells from RedMUC298trTg and Spdef -/-RedMUC298trTg were sorted to analyse the differential gene expression in the absence of Spdef. Overall design: Colonic GCs and the remaining epithelial cells (ECs) of the RedMUC298trTg and Spdef -/-RedMUC298trTg mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of mCherry signal. Sorted GCs were analysed using RNA sequencing (RNAseq).