LC-MALDI MS-based identification of other interesting proteins proteins from MVs derived from acrosome reacted porcine sperm.

Other interesting proteins identified in the MVs derived from bicarbonate capacitated and acrosome reacted cells based on similar criteria to Table 1. These are proteins that have been previously reported to have roles in secondary sperm-zona binding functioning. Proteins that were not detected in the capacitated membrane cavitates (Table 3) and are exclusively present in the MVs based on these analyses are highlighted in grey. Most of these proteins have been previously reported to be acrosomal membrane specific [29]. Protein ID was considered to be conclusive when two or more peptides were identified (eTable 2).Protein ID was considered conclusive when two or more peptides were identified (e*: indicate acrosomal specific membrane proteins.Highlighted in grey: Proteins that are not detected in the capacitated membrane cavitates and are exclusively present in the mixed vesicles.Highlighted in bold: SNARE interacting protein.