Mapping-by-sequencing of the eno mutation

To identify the mutation that underlies the ENO locus, we performed mapping-by-sequencing on an F2 population derived from the cross between an eno mutant and a wild tomato Solanum pimpinellifolium plant (accession LA1589). Genome-wide analysis of the allele frequencies in two pools containing 35 mutant and 50 randomly selected wild-type plants allowed us to identify the genomic region harboring the causal mutation of the eno phenotype.