Differential analysis of transcriptome of Trichophyton mentagrophytes treated with Trichoderma

Under the action of Trichophyton 1000 UG / ml, the colony of Trichophyton mentagrophyte was completely inhibited. The spore number and germination rate of Trichophyton mentagrophyte under the action of 100ug / ml and 10ug / ml were significantly lower than those in the control group. Under the action of Trichophyton, the mitochondria in the mycelium of Trichophyton mentagrophyte were cleaved. Under the action of trichomycin, the related genes in mitochondria decreased significantly. This showed that mitochondria were obviously damaged during trichomycin treatment. It is speculated that Trichophyton can cause mitochondrial damage and reduce the efficiency of respiratory chain, but Trichophyton can synthesize enough ATP by regulating related ATP synthase to resist the invasive effect caused by Trichophyton stimulation. Overall design: SDA drug sensitive plates with Trichoderma concentration of 10 and 100 UG / ml were prepared, respectively. The fungal cake was prepared by the method of fungal cake drilling and cultured on the drug sensitive plate for 6 days. Then collect the colonies and store them in liquid nitrogen. Perform the next step of high-throughput sequencing.