Integrative profiling of cells undergoing replicative and DNA-damage-induced senescence in human lung fibroblasts WI38. Homo sapiens

Cellular senescence (CS) is an adaptive stress response and is characterized by a robust proliferative arrest as well as a secretory phenotype (SASP). Senescent cells play physiological and pathophysiological roles. Despite advances in the understanding of senescence in physiology and disease, as well as interest in senescent cells as targets for anticancer and tissue regeneration therapies, our understanding of the fundamental regulation of the senescent state remains fragmentary. Further, the dynamic interactions occurring at the epigenomic level and the gene-regulatory networks underlying the CS transcriptional program are unknown. In order to reconstruct the dynamic gene regulatory program underlying the OIS cell fate transition, we performed time-series experiments for replicative and DNA-damage-induced senescence in WI38 fibroblasts.