Morphology and molecular phylogeny of <i>Coolia tropicalis, Coolia malayensis</i> and a new lineage of the <i>Coolia canariensis</i> species complex (Dinophyceae) isolated from Brazil

The morphology and molecular phylogenetic relationships of <i>Coolia</i> species isolated from Brazil, including <i>C. malayensis, C. tropicalis</i> and a new lineage of the <i>Coolia canariensis</i> complex, are presented. Morphology was investigated by light and scanning electron microscopy. No significant morphological differences between the Brazilian strains and other geographically distant strains of <i>C. malayensis</i> and <i>C. tropicalis</i> were observed. In LSU and ITS rDNA phylogenies <i>C. malayensis</i> and <i>C. tropicalis</i> sequences from Brazil branched within the clade of these species with strains isolated from elsewhere. Phylogenetic relationships (LSU rDNA) within the <i>C. canariensis</i> species complex support the existence of three cryptic species. No morphological difference was discernible among species in this complex. Additional molecular, morphological and toxin data from globally distributed isolates should be acquired to strengthen our knowledge of the <i>C. canariensis</i> species complex.本研究报道了从巴西分离得到的卡尔藻属（Coolia）物种的形态学特征与分子系统发育关系，涉及马来亚卡尔藻（C. malayensis）、热带卡尔藻（C. tropicalis）以及加那利卡尔藻复合群（C. canariensis complex）的一个新支系。本研究通过光学显微镜与扫描电子显微镜对其形态学特征进行了观测。结果显示，巴西分离株与其他地理区域来源的马来亚卡尔藻和热带卡尔藻菌株之间无显著形态学差异。在大亚基（LSU）rDNA与内转录间隔区（ITS）系统发育分析中，来自巴西的马来亚卡尔藻和热带卡尔藻序列与其他地区分离株一同聚类于该物种的进化支内。基于大亚基rDNA的加那利卡尔藻复合群系统发育关系分析结果，支持该复合群存在3个隐存物种的结论。该复合群内各物种之间未观测到可区分的形态学差异。未来应采集全球范围内各分离株的更多分子、形态学及毒素相关数据，以深化对加那利卡尔藻复合群的认知。

搜集汇总

背景与挑战

背景概述

该数据集总结了巴西分离的Coolia tropicalis、Coolia malayensis和Coolia canariensis物种复合体新谱系的形态学和分子系统发育研究。研究发现巴西菌株与全球其他菌株在形态上无显著差异，且系统发育分析支持C. canariensis复合体存在三个隐存物种，但形态无法区分。研究强调了获取更多全球数据以深化对C. canariensis物种复合体理解的重要性。