RNA-sequencing of THP-1 derived macrophages with and without ASAP2 knockdown

To explore the potential functions of ASAP2 in macrophage, we established ASAP2 stable knockdown THP-1 cells and differentiated them into macrophages with PMA (50 ng/ml, 48 hours). Then we performed RNA-sequencing on the macrophages derived from THP-1 with (shASAP2) or without ASAP2 knockdown (shNC) to analyze the enriched functions by ASAP2-correlated genes and differentially expressed genes. THP-1 derived macrophage (THP-1 cells were differentiated into macrophage in a 6 cm dish by 50 ng/ml PMA for 48 hours). Total RNA was extracted from shNC (n=3) and shASAP2 (n=3) THP-1 macrophage. Illumina novaseq 6000 platform was used to sequence the RNA samples for the subsequent generation of raw data.