Transcriptomic profile of single-cell sorted monocytes, T cells and CD3+CD14+ cells from one subject infected with latent tuberculosis

We set out to detect a transcriptional signature in CD4 T cells of individuals at risk of progression to active tuberculosis. We indeed found such a signature which surprisingly could be tracked to a cell population expressing both T cell (CD3) and monocyte (CD14) markers. To identify the nature of the CD3+CD14+ population, we determined the transciptomic profile of single cells CD3-CD14+ Monocytes (n=21), CD3+D14- T cells (n=22), CD3+CD14+ cells in the Monocyte gate (Mgate) (n=22) and CD3+CD14+ cells in the lymphocyte gate (Lgate) (n=20) from one subject infected with latent tuberculosis by RNA sequencing. PCA on all genes showed that CD3+CD14+ cells represent an intermediate population between Monocytes and T cells, with CD3+CD14+ L gate cells closer to T cells compared to CD3+CD14+ Mgate cells. Overall design: Single cell RNA-sequencing of sorted singlets monocytes (CD14+CD3-), singlets T cells (CD14-CD3+), CD3+CD14+ cells (Lgate or Mgate) from one subject infected with latent tuberculosis.