Distinct lung functional, histological and cell senescence signatures in the single vs repetitive bleomycin mouse model of idiopathic pulmonary fibrosis

To address the gap between acute bleomycin-induced fibrosis in mice and the chronic nature of human IPF, we performed RNA-seq on lung tissue from single-dose and repetitive bleomycin models of idiopathic pulmonary fibrosis (BLEO-IPF). We find that the repetitive BLEO-IPF model recapitulates the key features of progressive fibrosis and senescence, offering a relevant pre-clinical platform for studying chronic IPF pathology and evaluating anti-fibrotic interventions. Overall design: We performed RNA-seq on poly(A)-enriched RNA isolated from the inferior lung lobes of four mouse cohorts: healthy controls (n = 20), mice with single-dose BLEO-IPF (n = 14), mice with repetitive-dose BLEO-IPF (n = 10), and a matched baseline group for the repetitive model (n = 9).