Identification of cleaved CTDs and their putative cleavage sites.

a The semi-tryptic peptides identified by MS/MS are shown underlined, together with the six amino acid residues immediately N-terminal to the proposed cleavage sites (/). b The Mascot score shown is for the underlined semi-tryptic peptide (shown in previous column). Scores above 35 were significant according to Mascot (p<0.05), however due to the consistent pattern of results, scores above 20 were deemed significant. c The total number of CTD peptides identified. d The peptide identified was from HagA since Kgp was not present in this mutant. e This Kgp sequence cannot be distinguished from an identical sequence found in the expected cleavage site sequence of HagA (PG1837). Identification of cleaved CTDs and their putative cleavage sites.