Cytometryczna analiza wpływu anestetyków na właściwości biologiczne komórek macierzystych z tkanki tłuszczowej

This readme file was generated on [2024-04-26] by [Malgorzata Maj]GENERAL INFORMATIONTitle of Dataset: Cytometric analysis of anesthetics influences on biological characteristics of adipose-derived stem cells.Author/Principal Investigator InformationName: Malgorzata MajORCID: https://orcid.org/0000-0003-2433-8578Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department of Tissue EngineeringAddress: Karłowicza 24, 85-092 Bydgoszcz, PolandEmail: [e-mail]/Associate or Co-investigator InformationName: Anna BajekORCID: https://orcid.org/0000-0002-7720-8542Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department of Oncology Address: Łukasiewicza 1, 85-821 Bydgoszcz, PolandEmail: [e-mail]/Alternate Contact InformationName: Nadia FatygaORCID: Institution: Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Department of Tissue EngineeringAddress: Karłowicza 24, 85-092 Bydgoszcz, PolandEmail: [e-mail] of data collection:  Geographic location of data collection: Information about funding sources that supported the collection of the data: This research received no specific grant from any funding agency.SHARING/ACCESS INFORMATIONLicenses/restrictions placed on the data: not applicableLinks to publications that cite or use the data: not applicableLinks to other publicly accessible locations of the data: not applicable Links/relationships to ancillary data sets: not applicableWas data derived from another source?If yes, list source(s): not applicableRecommended citation for this dataset: not applicable DATA & FILE OVERVIEWFile List: 72h inkubacji kontrola_2_CD34_13062016143339.pdf72h inkubacji kontrola_2_CD44_13062016143315.pdf72h inkubacji kontrola_2_CD45_13062016143349.pdf72h inkubacji kontrola_2_CD90_13062016143327.pdf72h inkubacji z mieszanina srodkow_2_CD34_13062016143426.pdf72h inkubacji z mieszanina srodkow_2_CD44_13062016143405.pdf72h inkubacji z mieszanina srodkow_2_CD45_13062016143436.pdf72h inkubacji z mieszanina srodkow_2_CD90_13062016143415.pdf48h inkubacji kontrola_2_CD34_13062016143143.pdf48h inkubacji kontrola_2_CD44_13062016143122.pdf48h inkubacji kontrola_2_CD45_13062016143157.pdf48h inkubacji kontrola_2_CD90_13062016143134.pdf48h inkubacji z mieszanina srodkow_2_CD34_13062016143231.pdf48h inkubacji z mieszanina srodkow_2_CD44_13062016143215.pdf48h inkubacji z mieszanina srodkow_2_CD45_13062016143252.pdf48h inkubacji z mieszanina srodkow_2_CD90_13062016143224.pdf24h inkubacji kontrola_2_CD34_13062016142847.pdf24h inkubacji kontrola_2_CD44_13062016142815.pdf24h inkubacji kontrola_2_CD45_13062016142920.pdf24h inkubacji kontrola_2_CD90_13062016142837.pdf24h inkubacji z mieszanina srodkow_2_CD34_13062016143011.pdf24h inkubacji z mieszanina srodkow_2_CD44_13062016142943.pdf24h inkubacji z mieszanina srodkow_2_CD45_13062016143031.pdf24h inkubacji z mieszanina srodkow_2_CD90_13062016142957.pdfRelationship between files, if important: not applicableAdditional related data collected that was not included in the current data package: not applicableAre there multiple versions of the dataset?If yes, name of file(s) that was updated: not applicableWhy was the file updated? When was the file updated? METHODOLOGICAL INFORMATIONDescription of methods used for collection/generation of data: Fluorescence measurements at different wavelengths provide quantitative and qualitative data about intracellular molecules such as DNA. Once the optics directs the light to a detector, the electronics system turns that light into an electronic signal. Each signal pulse represents an individual event (or cell). Cytometers display data in terms of height, width, and area, describing the properties of the signal pulse.Methods for processing the data: BD FACSDiva 8.0.1 software offers efficient data management tools to simplify data storage and improve flow cytometer data organization. It provides automated data export in XML, FCS, and even ZIP formats. Manual export of FCS files creates an experiment folder structure in the operating system for easy identification of data files without the need to open them. While FCS files are widely used, they can be challenging due to their proprietary format. For that reason, they were converted to PDF.Instrument- or software-specific information needed to interpret the data: BD FACSDiva™ Software 8.0.1 provides a wide array of basic analysis features enabling multiple applications to run on the BD FACSCanto Flow Cytometers.Standards and calibration information, if appropriate: Cytometer settings are tracked over time by Levey-Jennings graphs to monitor cytometer performance. Adjustments are made automatically to ensure consistent data integrity and reproducible results from day to day and experiment to experiment. Environmental/experimental conditions: Measurements were performed at standard conditions. Describe any quality-assurance procedures performed on the data: Integrated quality control features further support data integrity by notifying operators if an assay fails predefined standards.People involved with sample collection, processing, analysis and/or submission: Maria Bugajska-Liedtke, Nadia Fatyga, Malgorzata Maj