Multimodal, quantitative analysis enables process management of productive, mixotrophic biofilms - supplemental flow cytometric data

The flow cytometric datasets of the two major experiments presented in the manuscript “Multimodal, quantitative analysis enables process management of productive, mixotrophic biofilms”. The cytometric measurements were performed using a CytoFLEX V3-B4-R0 analyser (Beckman-Coulter, Brea, USA). All utilized channels were induced by the blue 488 nm laser. The forward scatter signal (FSC, 488 nm ± 5 nm, gain 123) is related to cell size and was tapped directly. The order of the other channels inside the wavelength division multiplexing assembly was optimized for best resolution trade-offs: 1) R. palustris fluorescence (780 ± 60 nm, gain 500), 3) P. taiwanensis_eGFP fluorescence (525± 40 nm, gain 200), 4) side scatter (SSC, 488 ± 8 nm, gain 59, trigger signal, threshold 1400). Slot 2 was not utilized and filled with a dummy filter reflective in the wavelength windows of channel 3) and 4). All parameters were used as height-based values. B51503, CytoFLEX Sheath Fluid (Beckman-Coulter, Brea, USA) was used in the fluidic system. The cytometer was operated using CytExpert v2.3.0.84 (Beckman-Coulter, Brea, USA).