Tet2 deficiency altered hematopoietic stem and progenitor cells ageing process

we compared self-renewal and lineage specification of WT and Tet2-deficient HSPCs during ageing and demonstrated that Tet2 ablation alters age-dependent HSC functional decline, revealing a disparate ageing process in the Tet2-deficient haemopoietic system. Overall design: We collected hematopoietic progenitor and stem cells (HSPCs) from young and aged WT/ Tet2KO/Dnmt3aKO mice for WGBS, 5hmC mapping (CMSIP), SMART-seq, H3K9me3 CHIP seq, and scATAC seq. We captured and compared the 3D genome structure among four groups ( WT-young, WT-old, T2KO-young and T2KO-old).