Crystal structure of radical SAM epimerase EpeE C223A mutant from Bacillus subtilis with [4Fe-4S] clusters, S-adenosyl-L-homocysteine and RiPP peptide 6 bound
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Crystal structure of radical SAM epimerase EpeE C223A mutant from Bacillus subtilis with [4Fe-4S] clusters, S-adenosyl-L-homocysteine and RiPP peptide 6 bound Descriptor: 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, IRON/SULFUR CLUSTER, ... Authors: Polsinelli, I, Fyfe, C.D, Legrand, P, Kubiak, X, Chavas, L.M.G, Berteau, O, Benjdia, A. Deposit date: 2022-07-25 Release date: 2024-01-10 Last modified: 2026-03-04 Method: X-RAY DIFFRACTION (2.15 Å) Cite: Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat.Chem.Biol., 20, 2024
来自枯草芽孢杆菌(Bacillus subtilis)的自由基S-腺苷甲硫氨酸(radical SAM)异构酶EpeE C223A突变体的晶体结构,该结构结合有[4Fe-4S]团簇、S-腺苷-L-高半胱氨酸以及结合的RiPP(核糖体合成并经翻译后修饰的肽,Ribosomally synthesized and post-translationally modified peptides)肽6。配体描述:1,2-乙二醇、二(羟乙基)醚、铁硫团簇等。
作者:Polsinelli I、Fyfe C.D、Legrand P、Kubiak X、Chavas L.M.G、Berteau O、Benjdia A
提交日期:2022-07-25
发布日期:2024-01-10
最后修改日期:2026-03-04
实验方法:X射线衍射(分辨率2.15 Å)
引用文献:自由基SAM酶催化RiPP异构化的结构与机制基础. Nat.Chem.Biol., 20, 2024
创建时间:
2022-07-25



