Characterizing Local Extension of Retinoblastoma by Single-cell Transcriptomics

Retinoblastoma (RB) is the most prevalent ocular tumor of childhood whose extraocular invasion largely increase metastasis risk. Nevertheless, a single-cell characterization of RB local extension has been lacking. Here, we perform single-cell RNA sequencing (scRNA-seq) 4 RB samples (2 from intraocular and 2 from extraocular RB patients), and integrate public scRNA-seq datasets to characterize the local extension of RB at single-cell level. Human eye samples were collected immediately following eye enucleation, tissue was dissected to isolate the tumor region for single-cell dissociation. we applied scRNA-seq on 4 RB samples (2 from intraocular RB patients and 2 from extraocular RB patients