16S_OTU_table.xlsx

Raw 16S rRNA OTU count table including nectar samples and controls.
Raw OTU table for long-read 16S rRNA amplicon sequencing of nectar microbiota. Columns correspond to individual libraries (nectar samples from NIL pairs 1 and 2, mock community controls, field controls, and water blanks), and rows correspond to OTUs clustered at 97% sequence identity. Values indicate the number of reads assigned to each OTU in each sample prior to filtering. Samples labelled “Mock” correspond to ZymoBIOMICS Microbial Community Standard controls processed in parallel through DNA extraction, library preparation, sequencing, and bioinformatic analyses. Samples labelled “Water” correspond to extraction blanks. Field control samples (e.g. empty capillaries exposed during sampling) are also included. This table underlies the calculation of read depth per sample, OTU richness, dominant OTU proportions, and subsequent filtering steps described in the Methods.