lncRNA changes in the livers of db/db mice than that of C57 mice

To identify putative lncRNA changes in the livers of db/db mice and C57 mice, we isolated the liver tissues and carried out a microarray analysis. Here, we found significant changes of lncRNAs in the livers of db/db mice. Among them, abnormal expression of ultraconserved elements was noticed. RNA was isolated from the mixed samples of liver tissues from 6 male db/db mice and 6 age-matched male wild-type (WT) mice. Total RNA was reverse-transcribed with biotin end-labeled random oligonucleotide primers. Then, the cDNA was hybridized to the Agilent mouse lncRNA+mRNA Array V1.0, including sense and antisense probes to all 481 human ultraconserved sequences, each spotted in duplicate.