Transcriptomic Analysis of Wheat Seedling Responses to the Systemic Acquired Resistance Inducer N-Hydroxypipecolic Acid

This experiment is to assess global changes of wheat gene expression in response to N-hydroxypipecolic acid (NHP), which is an inducer of plant systemic accquired resistance. Six plates (24 seeds per plate) of wheat (Triticum aestivum) cultivar Zhongyuan 98-68 were planted under 25ºC incubator. After three days, three plates of seedlings were treated with 1 μL 1 mM NHP per seedling, called as NHP-rep 1,2,3. The other three were treated with water and used as the control group (called as water-rep 1,2,3). The coleoptiles were collected after one day after the treatment. Each plate serves as a biological replicate for its treatment group for RNA sequencing. Three biological replicates of four-day-old wheat seedling at one day post water treatment were named water-rep 1, 2, 3, used as control. Three biological replicates of four-day-old wheat seedling at one day post NHP treatment (1 μL 1mM NHP per seedling) were named NHP-rep 1, 2, 3. Total RNA extracted from water-rep 1, 2, 3 and NHP-rep 1, 2, 3 were used for library preparation. The RNA sequencing was performed at Sinotech Genomics. The libraries for sequencing were prepared using SureSelect Strand-Specific RNA Component kit. RNA sequencing was performed using an Illumina NovaSeq 6000 machine to obtain 150 nt paired-end reads.