aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing
VCQA = Vector Control Quantitative Analysis; E = efficiency of amplification; CT = number of cycles.Quantitative analysis of DNA purity using VCQA employing qPCR.
We performed the expression microarray experiments for miRNA of rectal cancer tissue. microRNA were extracted from primary tumor of 137 rectal cancer patients, hybridized and scanned with Affymetrix m
aUnderlined sequences are restriction enzymes. Bold sequence represents the histidine tag.* Negative PCR for B. ovis mutant strain.&Negative PCR for TT17573 strain.Primers for PCR used in this study.
