Innovative Reproductive Management in Rabbits GnRH-Free Insemination with Specifically Designed Cannulas for Nulliparous and Multiparous Does.

This dataset encompasses research data evaluating the efficacy of artificial insemination (AI) in rabbits using two distinct prototype insemination cannulas specifically adapted to the animal's physiological status (nulliparous vs. multiparous). The experimental design focused on inducing ovulation through mechanical stimuli without the exogenous administration of GnRH. The AI procedures included a comparative analysis of two different methods—one versus two cannula insertions—performed by two distinct operators to account for procedural and inter-individual variability. Furthermore, the repository contains a detailed comparative neuro-anatomical study of the caudal vagina, specifically focusing on the most distal six centimeters. This morphological analysis integrates the quantification of mechanoreceptors to evaluate sensory density across the vaginal wall, the immunohistochemical identification of Merkel cells utilizing Cytokeratin 20 (CK20) as a specific molecular marker for high-resolution detection, and the systematic identification and morphometric measurement of nerve ganglia to provide quantitative data on the peripheral nervous system's involvement in this region.