Experiment Design.

Overview of the experiment for each DENV-2 strain. For each strain and concentration of JNJ-1802 tested, 16 plates were seeded with Vero cells at a density of 5,000 cells/well. Six wells were used to examine viral kinetics in the absence of JNJ-1802, and four wells were used to examine the viral kinetics in the presence of JNJ-1802 (concentration range: 0.00256 nM-8 nM .24 Hours after seeding, the cells in each well were infected with DENV-2). For 10 of the 16 plates, the medium was not refreshed during the experiment and each day for 10 days, one of these plates was further processed. For the other 6 plates, the medium was refreshed at day 4 post infection and from day 5 onwards, one plate was harvested each day until day 10.