Expression data from LSK WT, GMP WT and GMP NcstnKO

Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic system, Notch is essential for the emergence of definitive HSC during fetal life and controls adult HSC differentiation to the T-cell lineage. Notch activation is controlled by the gamma-secretase complex complex, composed of presenilin, nicastrin (Ncstn), anterior pharynx-1 (Aph1), and presenilin enhancer-2 To determine other role of Notch signaling in HSC we designed a conditional mouse model of Nicastrin deletion. WT and KO HSC were analysed to determined genes controlled by Notch signaling. Bone marrow lineage negative, cKit+, Sca1+ and lineage negative, cKit+, Sca1- CD34+ FcgammaRII/III+ cells were sorted from WT and Ncstn KO littermates for RNA extraction and hybridization on Affymetrix microarrays