H460-RUNX3.xlsx

To identify genes regulated by RUNX3, we performed mRNA sequencing (RNA-seq) in H460-ERT2-RUNX3 and H460-ERT2-RUNX3-K94/171R cells. H460-vec, H460-ERT2-RUNX3, and H460-ERT2-RUNX3-K94/171R cells were serum-starved for 24 hr, and then stimulated with 10% serum or 10% serum + 1mM 4-OHT for 0, 8, or 16 hr. RNA was extracted from the cells, and gene expression patterns were analyzed by mRNA sequencing. Expression of genes 8 or 16 hr after serum stimulation was quantified as log2(fold change) relative to the average of control reactions (i.e., before serum stimulation, 0 hr) for each cell line.