Dietary protein and lysosome rich enterocyte (LRE) activity impact zebrafish microbiome

The goal of this study was to measure the effects of dietary protein and lysosome rich enterocyte (LRE) activity on the zebrafish microbiome. We tested the combined effects of the cubilin (cubn) mutation and custom-formulated, isocaloric high and low protein diets on the larval zebrafish microbiome. We fed high-protein (HP) or low-protein (LP) diets to cubn heterozygote and homozygous mutant siblings from 6 to 30 dpf, then performed 16S rRNA gene sequencing on whole larvae to identify microbial populations.