Transcriptome from the cerebral cortex of 5xFAD mice with neuronal expression of a T249A/T251A mutant form of mouse E2F4 (E2F4DN) vs a control protein (EGFP)

Alzheimers disease (AD) has a multifactorial etiology, which requires a single multi-target approach for an efficient treatment. We have studied the putative role of E2F4 in AD, as this transcription factor regulates cell quiescence and tissue homeostasis, controls gene networks affected in AD, and is upregulated in the brain of AD patients and of APP/PS1 and 5xFAD transgenic mice. E2F4 contains an evolutionarily-conserved Thr-motif that, when phosphorylated, modulates its activity (//doi.org/10.1128/MCB.00239-12), thus constituting a potential target for intervention. We have generated transgenic mice with neuronal expression of a dominant negative form of E2F4 lacking this Thr-motif (E2F4DN), which was crossed with 5xFAD mice. Then, the brains of their descendants and of control EGFP/5xFAD mice (2 per genotype) were subjected to transcriptomic analysis.