Endothelial KLF15/VASN axis inhibits angiogenesis via activation of Notch1 signaling [ATAC-Seq]

Kruppel like factors (KLFs) are a subfamily of zinc finger proteins. As one of the family members, KLF15 can specifically bind to GC rich sequences on the promoter, thereby exerting transcriptional regulatory effects. Here we found that KLF15 negatively regulates angiogenesis in endothelial cells. KLF15 induces an increase in vasorin (VASN) expression, thereby activating the Notch signaling pathway and leading to a decrease in angiogenesis. These findings point to an KLF15/VASN axis as critical determinants of Notch signaling activation. Overall design: ATAC-seq of endothelial cells transfected with control adenovirus or adenovirus carrying KLF15 gene