Integrated genome-wide investigations of the housefly, a global vector of diseases reveal unique dispersal patterns and bacterial communities across farms. GBS and Microbiome Musca

Adult houseflies, (Musca domestica L.) lives in intimate association with various numerous microorganisms and are vectors of human pathogens. In temperate areas houseflies will overwinter in environments constructed by humans and recolonize surrounding areas in early -summer. However, the dispersal pattern and associated bacteria across seasons and locations are unclear. In order to better understand the variation and association between bacteria communities and the housefly across farms, we used 16S rRNA gene amplicon sequencing to describe bacterial communities. Secondly, we used genotyping-by-sequencing (GBS) for the simultaneous identification and genotyping of thousands of Single Nucleotide Polymorphisms (SNPs) to establish dispersal patterns of individual houseflies across farms. In total 400 individual flies were sampled within and between 11 dairy farms in Denmark.